The capacity of the colon to function properly is harmed by a variety of conditions. Some of these include Crohn’s disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD). Ulcerative colitis which is the most common type of IBD. It's a relapsing and remitting disorder with a wide range of phenotypic and genotypic characteristics. In this study, potential protective and curative effect of probiotics on acetic acid-induced ulcerative colitis was evaluated. Ulcerative colitis was induced in rats by 2 ml of 3% glacial acetic acid (v/v) administered intracolonially for 3 successive days. Forty male albino rats were divided into five equal groups. Group I: (Control normal group) rats received no drugs. Group II: Early UC-induced group, Group III: Late UC-induced group, Group IV: (Early UC + probiotics protected) rats received probiotics (135 mg/kg b. wt./day) for 21 days prior to acetic acid administration. Group V: (Late UC + probiotics treated) rats first administered with acetic acid then after 3 days probiotics was received for 21 days. A significant increase in L-MDA and Myeloperoxidase (MOP) and up-regulation of IL-1β, caspase-3 with down-regulation in Bcl-2 gene expression as well as marked decrease in GSH and CAT were observed in colon tissue of UC-induced rats. However, administration of probiotics to UC-induced rats caused a marked improvement and reverses all previous parameters near the average range. In conclusion, probiotics protected colonic mucosa against UC and may be effective in enhancing the healing of ulcerated colon via anti-inflammatory and anti-apoptotic activities and regenerating endogenous antioxidant mechanism. |
