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Prof. Samy Ali Hussein Aziza :: Publications:

Title:
Caspase-3, Bcl-2, p53, CYP1A1 and COX -2 as a potential target in chemoprevention of Benzo (a) pyrene-induced lung carcinogenesis in mice: Role of thymoquinone
Authors: Samy Ali Hussein , Abdel-Aal, S.A , Aziza Amin and Heba Atef Khalaf
Year: 2016
Keywords: Thymoquinone, Benzo(a)pyrene, Bcl-2, COX-2, P53, Caspase-3, CYP1A1, Lung cancer
Journal: International Journal of Chemical and Natural Science
Volume: 4
Issue: 3
Pages: 430-441
Publisher: Aizeon publishers
Local/International: International
Paper Link:
Full paper Samy Ali Hussein Aziza_ijcns430-441.pdf
Supplementary materials Not Available
Abstract:

Benzo[a]pyrene [B(a)P], a well-known environmental carcinogen, promotes oxidative stress and DNA damage. Thymoquinone (TQ) exhibits promising effects against inflammatory diseases and cancer. The possible protective and chemopreventive effects of TQ against [B(a)P] -induced lung cancer in mice were investigated. One hundred male Swiss albino mice divided into four equal groups. Group Ι: (Control) received no drugs. Group Π:(lung cancerinduced) mice injected with a single dose of [B(a)P] (100 mg/ kg b.wt, i.p). Group III: (lung cancer + TQ treated) mice injected with [B(a)P] as in group II and treated with TQ (20 mg/kg b.wt/day, orally) from 22th week to 30th weeks. Group IV: (lung cancer + TQ protected) mice received TQ (20 mg/kg b.wt. / Orally) on alternate days from 1 day prior to [B(a)P] injection and were treated continuously with TQ until 30th week. Blood samples and lung tissue for determination of serum CEA, Haptoglobin (HPT), ADA and ɤ-GT in addition to enzymatic antioxidants status (CAT, SOD, GPx, GST and GR), L-MDA, NO, GSH, Bcl-2, CYP1A1, P53, Caspase 3, DNA fragmentation and COX-2 in lung tissues. The obtained results revealed that, [B(a)P] potentially increased serum CEA, HPT, ADA, ɤ- GT in addition to COX-2, Caspase 3, L-MDA, NO, Bcl-2, CYP1A1, P53, and DNA fragmentation in lung tissues. However, antioxidant enzymes activities and GSH were markedly decreased. It could be concluded that, TQ mitigate lung cancer through its radical scavenging activity and anti-inflammatory effect, regenerating endogenous antioxidant mechanisms and attenuated the increased caspase 3, DNA fragmentation, COX-2, Bcl-2, CYP1A1, P53 and oxidative stress in lung tissues. These results suggest that, the possible efficiency of TQ as a distinct chemo-preventive agent in lung carcinogenesis.

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