The present study was carried out on 105 random samples of frozen beef burgers, collected from different localities (Benha city, centers
and villages with 35 for each locality) at Kaliobia Governorate, to evaluate the bacterial quality and the hygienic health hazard of
them with some food borne pathogens. The bacteriological examination of samples collected from, Benha city, centers and villages
revealed that, the mean value of APC, Psychrotrophic, Enterobacteriaceae, Coliform and Staphylococcus counts in frozen beef burger
samples collected from Benha city were 2.97 × 104 ± 0. 15 × 104; 1.76 × 104 ± 0. 11 × 104; 1.35 × 102 ± 0. 11 × 102; 0.73 × 102 ± 0.18 ×
102 and 1.34 × 102 ± 0.09 × 102, respectively; for samples collected from different centers were 4.74 × 104 ± 0.17 × 104; 2.32 × 104 ±
0.10 × 104; 1.74 × 102 ± 0.10 × 102; 1. 29 × 102 ± 0.09 × 102 and 1.88 × 102 ± 0.10 × 102, respectively; for samples collected from different
villages were 7.91 × 104 ± 0.16 × 104; 5.21 × 104 ± 0.19 × 104; 3.36 × 102 ± 0. 17 × 102; 2. 23 × 102 ± 0.13 × 102 and 2.38 × 102 ±
0.10 × 102, respectively. 14 isolates of E. coli were isolated from examined frozen beef burger samples collected from different localities
(Benha city, centers and villages) represented as 2(5.7%) from samples of Benha city with serotypes one O55:K59(B5) and one
O125:K59(B5); 5 (14.3%) from samples of centers with serotypes 2 O55:K59(B5), 2 O125:K59(B5) and one O126:K71 (B16) and 7
(20.0%) from samples of villages with serotypes 3 O55:K59(B5), 2 O125:K59(B5), one O111:K58(B9) and one O126:K71(B16). In
addition, 19 isolates of Coagulase positive S. aureus were isolated, 3 (8.6%) from samples of Benha city; 6 (17.1%) from samples of
centers and 10 (28.6%) from samples of villages. SET- RPLA test revealed that, 6 strains out of 10 randomly examined strains (60.0%)
were enterotoxigenic and classified according to type of toxin into (3A; 1C, 2A and C). Ps. aeruginosa strains were the only species
isolated from examined samples and three beef burger samples collected from different villages were only positive for their isolation.
Moreover, the present study failed to detect Aeromoneus species and Salmonella serovars from all examined frozen beef burger
samples. |
