The present study was prepared to investigate fungi associated within Co-occurrence of
aflatoxins (AFs) andochratoxin A (OTA) in some cereals using High Performance Liquid
Chromatography (HPLC) to compare the levels of contamination with the Egyptian
standard (ES) and European unions (EU) regulation. Sixty cereals samples (white corn,
yellow corn, wheat and polished rice) were collected from different localities in Qalubyia
governorate. The results indicated that Total Fungal count (TFC) isolated were 535 isolates
in non-sterilized cereals and 276 isolated with sterilized cereals. Also data showed that
the most isolated fungal species were identified as. Aspergillus, Penicillium, Fusarium,
Rhizopus, Alternaria and Cephalosporium Allergy. It was observed that Aspergillus spp. (A.
flavus, A. niger and A. parasiticus) were the most frequent in all samples. When testing the
ability of isolated fungi especially Aspergillus flavus, Aspergillus parasitic us to produce for
AFs as well as Aspergillus niger and Aspergillus ochraceous for OTA. Results clarified 24
isolates of A. flavus and10 isolates of Aspergillus parasitic us were producers for AFs. On
the other hand the obtained results indicated that 17 and 3isolates of Aspergillus niger and
Aspergillus ochraceous were producers for OTA, respectively. The result indicated that the
percentages of the incidence of AFs in samples were 60%, 46.6%, 26.6% and 33.3% with
white corn, yellow corn, wheat and rice, respectively. Accordingly to results six samples
exceeded the maximum levels of AFs set in the EU and ES. While all the rice samples under
study were within the permissible limits as recommended by the ES and EU. On the other
hand the incidence of OTA in cereals samples are 40%, 26.6%, 20%and 20% of cereals
samples (yellow corn, wheat, rice and white corn), respectively. According to obtained
results OTA in samples were less than the regulatory limits as recommended by ES and EU. |
