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Ass. Lect. Wagihah rashad moselhy eldmrdash :: Publications: |
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| Title: | 1. Characterization of an archaeal photoreceptor/transducer complex from Natronomonas pharaonis assembled within styrene–maleic acid lipid particles |
| Authors: | N. Voskoboynikova, †a W. Mosslehy, †a A. Colbasevici, a T. T. Ismagulova, b D. V. Bagrov, b A. A. Akovantseva, d P. S. Timashev, cd A. Y. Mulkidjanian, a V. N. Bagratashvili, d K. V. Shaitan, b M. P. Kirpichnikovb and H.-J. Steinhoff. |
| Year: | 2017 |
| Keywords: | Not Available |
| Journal: | RSC Advances |
| Volume: | 7 |
| Issue: | 51324 |
| Pages: | Not Available |
| Publisher: | Royal Society of Chemistry |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | Wagihah rashad moselhy eldmrdash_Characterization_of_an_archaeal_photoreceptortrans.pdf |
| Supplementary materials | Not Available |
| Abstract: |
The styrene–maleic acid (SMA) copolymers enable a detergent-free extraction of membrane proteins from lipid bilayers yielding stable water-soluble nanocontainers with lipid-encased proteins. Here, we demonstrate the ability of the SMA copolymer to extract a complex of interacting membrane proteins from proteoliposomes, namely the archaeal sensory rhodopsin II from Natronomonas pharaonis, NpSRII, in complex with its transducer, NpHtrII. The NpSRII/NpHtrII complexes retained their structural integrity within SMA lipid particles as revealed by diverse biophysical techniques. Pulse electron paramagnetic resonance data showed the SMA-encased NpSRII/NpHtrII complexes as native-like 2 : 2 dimers with a distance between NpSRII sites 159 according to the “V”-shaped conformation found in a crystal structure. |
