Background and methodology: Brucellosis in livestock causes huge economic loses of developing countries and
demonstrates a serious health risks to the patrons of infected dairy goods and meat. Prevention of brucellosis in
cattle relies on the trustworthiness of the techniques utilized to detect the causative pathogen. In the current
investigation, we described the exploration of the well-known real-time PCR technique based on the Brucellaspecific IS711 with different tissue samples of dromedary camels in Qatar.
Results: The findings of the real-time PCR unveiled the occurrence of Brucella spp in 60 % of lung tissues, 71.42 %
of liver tissues, 72 % of spleen tissues, 25 % of kidney tissues, and 42.42 % of placental fluid samples. Among
them, the liver tissues and spleen tissues possessed the highest number of positive results, whereas kidney tissues
displayed the lowest number of positive results for Brucella spp.
Conclusion: The findings of this investigation discloses that PCR technique is a sensitive and specific technique for
the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of
Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify
specific species of Brucella. |
