Dr. Samar Fawzy Gad :: Publications:

Background: Bisphenol A (BPA) is among the most industrial compounds worldwide. BPA belongs to a group of compounds that are categorized as endocrine disruptive chemicals resulting in multi organ toxicities. Garlic, a natural plant, has a recorded history in ancient medicine. Royal Honey possesses antioxidant and anti-inflammatory properties against many contaminants. Aim: to compare effect of aged garlic extract (AGE) and royal honey (RH) on protection against gastric mucosal damage by Bisphenol A consumption. Materials and Methods: For the study, four equal groups of forty rats were established. The control group had ten rats. For 28 days, the remaining thirty rats were given an oral dosage of 50 mg/kg of bisphenol A; ten rats of whom were assigned to group II. Other ten rats (group III) were pretreated with AGE (200 mg/kg) daily orally one hour before Bisphenol A. whereas the remaining ten rats (group IV) were pretreated with RH (250 mg/kg) daily orally one hour before Bisphenol A. The stomach was investigated using Haematoxylin and Eosin, a scanning electron microscope, Periodic Acid Schiff stain, caspase 3 and TNF- α immunostaining. Results: Bisphenol A treated group showed cell degeneration and erosions on the epithelial surface. The cytoplasmic reactivity of TNF- α and caspase 3 immunostaining is elevated, indicating a malfunction in the gastric mucosa. The pretreatment of AGE and RH mitigates the histopathological alterations with a significant decrease in TNF- α and caspase 3 immunreactivity. Conclusion: This study highlights the protective effects of natural compounds (AGE and RH) against BPA-induced gastric mucosal damage by reducing inflammation and apoptosis markers.