Background: Carbon Tetrachloride is used as a dry-cleaning solvent and in lava lamps. Previous research has
demonstrated its harmful potential for several organs. Aim: The objective of the current work is to examine any
hepatoprotection induced by Riboflavin, Urolithin A, and combined Riboflavin and Urolithin A on an animal model of
liver damage brought by CCl4. Histological and immunohistochemical investigations were also used to study the
processes behind such protection. Material and methods: Five equal groups of forty Wistar albino rats were created.
In this study, Wistar rats were given CCl4 intraperitoneally (1 mL/Kg bw) on day 7 as a single dose while given
riboflavin by oral gavage, Urolithin A intraperitoneally and combined Riboflavin and Urolithin A for 14 days. After the
experiment, blood samples were obtained to measure the liver enzymes. Liver samples were prepared using Masson
trichrome as well as Hematoxylin and Eosin staining. Bax and Bcl2 immunoexpression were detected.
Results: Along with disturbed architecture and vacuolization of hepatocytes shown in the H & E results linked with
fibrous propensity in the Masson- trichrome samples, CCl4-exposed animals demonstrated significant disruption of the
biochemical tests. In the group receiving either riboflavin or/and urolithin A, the histology and biochemical markers
were adjusted after therapy.
Conclusion: Through elevation of anti-apoptotic proteins and suppressing of pro-apoptotic proteins, riboflavin and/or
urolithin A protected against CCl4-induced liver damage. Together, they had a synergistic effect. |
