The synthesized pyrazolopyrimidine derivatives conjugated with selenium nanoparticles were prepared via a
reaction of pyrazolone 1 with aryl-aldehyde and malononitrile or 3-oxo-3-phenylpropanenitrile in the presence
ammonium acetate or pipridine using an ultrasonic bath as a modified method in the organic synthesis for such
materials. The structure of the synthesized compounds was elucidated through various techniques. All the
synthesized pyrazolopyrimidines were used in the synthesis of selenium nanoparticles (SeNPs). These nano-
particles were confirmed using UV-spectra, Dynamic Light scattering and (TEM) techniques. The larvicidal ef-
ficiency;of the synthesized;compounds; was investigated against some strains such as Culex pipiens;and Musca
domestica larvae. Bioassay test showed pyrazolopyrimide derivatives to exhibit an acceptable larvicidal;bio-
efficacy. The derivative (3) exhibited;the highest;efficiency for more than; lab strains of both species. More-
over, C. pipiens larvae were more sensitive towards the examined compounds than M. domestica. The field;strain
displayed lower affinity for the 2 folds compounds. Some biochemical changes were tracked through analysis of
insect main metabolites (protein, lipid and carbohydrate), in addition to measuring the changes in seven enzymes
after treatment. Generally, there was a reduction in the protein, lipids and carbohydrates after treatment with all
tested compounds. Moreover, a decrement was noticed for acetylcholine esterase and glutathione;S-transferase;
enzymes. There was an increment in the acid;phosphatase; and alkaline phosphatase. In addition, there was
elevation in Phenoloxidase level but it noticed the declination in both Cytochrome P450 and Ascorbate perox-
idase activity after treatment both flies with derivatives of selenium-nanoparticles in both lab and field strain.
Generally, the experiments carried out indicate that antioxidant and detoxification enzymes may play a signif-
icant role in mechanism of action of our novel nanocompounds. The cytotoxicity of the synthesized compounds
and conjugated with SeNPs showed enhanced compatibility with human normal fibroblast cell line (BJ1) with no
toxic effect. |
