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Prof. Nahla El-eraky El-azab El-eraky :: Publications:

Title:
Histological and immunohistochemical study of the potential therapeutic impacts of bone marrow mesenchymal stem cells and exosomes for sciatic nerve crush injury model in rats
Authors: Nahla El-Eraky El-Azab, Abeer M. El-Mahalaway, Ola Mostafa & Dina Sabry
Year: 2018
Keywords: Bone marrow mesenchymal stem cells; crush injuries; exosome; sciatic nerve
Journal: Journal of Histotechnology
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Not Available
Supplementary materials Not Available
Abstract:

ABSTRACT The aim was to evaluate the potential effect of bone marrow-derived mesenchymal stem cells (BMSC) and BMSC-EX in the sciatic nerve injury. Forty-five rats were divided into four groups, i.e. Group I control no surgery or treatment, Group II sciatic nerve crush surgery and 1 week postsurgery, Group III sciatic nerve crush injury was treated with BMSC, and Group IV sciatic nerve crush injury was treated with BMSC-EX. All animal groups were euthanized 2 weeks after treatment. Sciatic nerves were examined histologically and immunohistochemically. Group II showed various histological changes, i.e. vacuolation, degeneration and loss of nerve fibers. Group II showed a significant increase in collagen fibers, but with significant reduction in osmium tetroxide (OsO4)-stained myelin sheaths and neurofilament immunostaining compared to Group I. Group II also revealed distortion and disruption of myelinated nerve fibers. Groups III and IV showed significant improvement of both histological and immunohistochemical changes in Group II. BMSC and BMSC-EX were shown to improve axonal regeneration.

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