Background: Bisphenol A (BPA) is among the most industrial
compounds worldwide. BPA belongs to a group of compounds
that are categorized as endocrine disruptive chemicals resulting
in multi organ toxicities. Garlic, a natural plant, has a recorded
history in ancient medicine. Royal Honey possesses antioxidant
and anti-inflammatory properties against many contaminants.
Aim: to compare effect of aged garlic extract (AGE) and royal
honey (RH) on protection against gastric mucosal damage by
Bisphenol A consumption. Materials and Methods: For the
study, four equal groups of forty rats were established. The
control group had ten rats. For 28 days, the remaining thirty rats
were given an oral dosage of 50 mg/kg of bisphenol A; ten rats of
whom were assigned to group II. Other ten rats (group III) were
pretreated with AGE (200 mg/kg) daily orally one hour before
Bisphenol A. whereas the remaining ten rats (group IV) were
pretreated with RH (250 mg/kg) daily orally one hour before
Bisphenol A. The stomach was investigated using Haematoxylin
and Eosin, a scanning electron microscope, Periodic Acid Schiff
stain, caspase 3 and TNF- α immunostaining. Results: Bisphenol
A treated group showed cell degeneration and erosions on the
epithelial surface. The cytoplasmic reactivity of TNF- α and
caspase 3 immunostaining is elevated, indicating a malfunction
in the gastric mucosa. The pretreatment of AGE and RH
mitigates the histopathological alterations with a significant
decrease in TNF- α and caspase 3 immunreactivity. Conclusion:
This study highlights the protective effects of natural compounds
(AGE and RH) against BPA-induced gastric mucosal damage by
reducing inflammation and apoptosis markers. |
