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Dr. Maysa Saeed Moustafa Elsayed :: Publications:

Title:
Expression level of micro-RNA-1, IL-5, IFNγ, and TNFα in adultonset asthmatic patients
Authors: Shaimaa M. Abo-Youssefa, Amira H. Allama, Mysa S. Mustafab, Hasnaa S. Abd el Hamidb, Amany K. Shahatb, Hany H. Moussac
Year: 2021
Keywords: Not Available
Journal: Not Available
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: Local
Paper Link: Not Available
Full paper Maysa Saeed Moustafa Elsayed_ejcdt , vol70, 215-222.pdf
Supplementary materials Not Available
Abstract:

Background and objective Asthma is an inflammatory process, where T lymphocytes cells, mainly the Th1/Th2 cells, participate in bronchial asthma’s pathogenesis and progression. Studying the inflammatory and genetic mediators’ expression levels, which control this inflammatory process like microRNAs, is essential to illustrate this disease’s pathogenesis. This work aimed to evaluate the levels of miRNA-1 and inflammatory mediators interleukin (IL)-5, interferon-γ (IFNγ), and tumor necrosis factor (TNF)-α in the peripheral blood of adult-onset asthmatic patients and their correlation to the disease severity. Patients and methods A case–control study was carried out on 69 participants, divided over two groups: group I was the asthmatic group, which included 49 patients with adult-onset bronchial asthma more than 18 years old, subdivided to group IA, with mild asthma (10 patients), group IB, with moderate asthma (19 patients), and group IC, with severe asthma (20 patients). Group II was the control group, which included 20 healthy individuals. IL-5, TNFα, and IFNγ were measured by enzyme-linked immunosorbent assay (ELISA) and relative quantification of MiRNA-1 expression was done by PCR. Results miRNA-1 decreased significantly in asthmatics and decreased more with increasing severity of the disease, with a significant positive correlation between miRNA-1 and both forced expiratory volume in first second and IFNγ, and meaningful negative relationship between miRNA-1 and both IL-5 and TNFα. The sensitivity and specificity of miRNA-1 in diagnosing asthma and detecting its severity were 100%. Conclusion miRNA-1 expression level is significantly decreased in adult-onset asthmatic patients. It decreases with increasing severity of the disease, with a positive correlation between miRNA-1 and plasma IFNγ but a negative relationship between miRNA-1 and both plasma IL-5 and TNFα

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