Analysis for some field crops, feed and food products found in the local markets of Egypt was applied for
GMOs detection using molecular technique based on PCR. DNA was extracted from samples including three
groups italic fresh samples (egg plant, guava, olive, mango, tomato, potato and orange), cereals and seed
samples (wheat, barley, broad bean, chickpea, corn, lentils, lima bean, soybean) and food products (bechmel,
beef, chicken soup, chips, feta cheese, indomie, jelly, popcorn and powder milk), followed by tests to ensure
purity of DNA samples using specific primers. GMO detection in the studied samples was conducted using
specific primers for the most commonly used genes in genetic transformation such as 35S promoter from
Cauliflower mosaic virus and Nopaline synthase terminator (T-Nos), using the following plasmids as positive
control: PGIIMH35S-g2ps1 and pBI221. Tests revealed existence of GMOs in some imported products such as
pudding, Jelly, popcorn and sauce due to the presence of 35S promoter and NOS terminator in soybean, while tests showed negative results in the local crops tested |
