| You are in:Home/Publications/Using Antibiotic Kanamycien and Estenrase Isozyme as Efficient methods for Detecting the Transformed DNA in Tomato interspecific hybridization. | |
Prof. Makhlouf Mohamed Mahmoud Bekhit :: Publications: |
|
| Title: | Using Antibiotic Kanamycien and Estenrase Isozyme as Efficient methods for Detecting the Transformed DNA in Tomato interspecific hybridization. |
| Authors: | Bekhit, M. M. M.;Abdel-Sabour, M. S.; El-Said, M. Fareda; Nazeem, H. R.; Hassan, A. M.; Mageid, A. A. and Refaat, M. H. |
| Year: | 1997 |
| Keywords: | Not Available |
| Journal: | The Egyptian Journal of Applied science (EJAS) |
| Volume: | 12 |
| Issue: | No. 9 |
| Pages: | 141-154 |
| Publisher: | Egyptian Society of Applied Science in collaboration with Zagazig University. |
| Local/International: | Local |
| Paper Link: | Not Available |
| Full paper | Makhlouf Mohamed Mahmoud Bekhit_Using Antibiotic Kanamycien and Estenrase Isozyme as Efficient methods for Detecting the Transformed DNA in Tomato interspecific hybridization..pdf |
| Supplementary materials | Not Available |
| Abstract: |
Higher percentages of survival calli were detected after transformation of the wild type DNA (LA: 385) on selective medium with 1000 mg/L kanamycien for two, three, four and five weeks in the three cultivated varieties; UC-97/3, Peto-86 and Castle Rock (treated and untreated with DNA) beside the wild type parent. For using esttease isozyme as a marker for success of interspecific hybridization.it was revealed that only a band at 0.5 was transferred to the transformed clones with DNA from wild parent to ensure transformation to the clones of parents. |
