Background: Intra-abdominal infections can be seriously
complicated to abdominal sepsis causing injury of various organs
in the abdomen. Lactoferrin could be utilized in combination with
Ceftriaxone to treat intestinal sepsis. Objective: to assess the
possibility of Lactoferrin to ameliorate induced intestinal sepsis
in adult male albino rats when used in combination with
Ceftriaxone. Materials and methods: Fifty male rats were
utilized in this study. The rats were divided into five groups:
Group I (Control), Group II (Abdominal Sepsis model group):
Rats received a single intraperitoneal injection of 1ml fresh fecal
suspension (FSI) at a 50 mg/ml concentration. Group III
(Abdominal Sepsis + Ceftriaxone group): Rats were treated with
an intramuscular injection of Ceftriaxone, Group IV (Abdominal
Sepsis + Lactoferrin group): Rats were treated with oral
Lactoferrin, Group V (Abdominal Sepsis + Ceftriaxone +
Lactoferrin group): Rats were treated with a combination of both.
Colon samples were obtained and processed for histological
examination. Results: Group II exhibited epithelial injury,
detachment, and shedding; some crypts were damaged,
obliterated, and lost parallelism; there was obvious depletion of
goblet cells; and the lamina propria was markedly infiltrated with
inflammatory cells. Furthermore, a significant increase in TNF-α,
COX-2, and Caspase-3 immunoexpression was detected. Groups
III and IV exhibited some histological attenuation, while group V
showed histological architecture with more improvement. |
