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Dr. Gamal Wareth Abdelaziz Mohamed :: Publications:

Title:
Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR
Authors: Wareth, G., Melzer, F., Tomaso, H., Roesler, U., Neubauer, H
Year: 2015
Keywords: Brucella abortus ; Cross-species transmission; Real-time PCR; Small ruminants
Journal: BMC Research Notes
Volume: 8
Issue: Not Available
Pages: 212
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Gamal Wareth Abdelaziz Mohamed_BMC Res.Not.pdf
Supplementary materials Not Available
Abstract:

Background: Brucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and camels. Results: In the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact. Conclusion: This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. Information provided in this study is important for surveillance program, because eradication programs and vaccination strategies may have to be adapted accordingly.

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