Background and aim: Myocardial damage is one of the most common pathological findings in the world. It is vital to locate a productive common defensive factor against the myocardial damage. The present investigation was embraced to assess cardio-protective action of aqueous extract of Moringa oleifera (M. oleifera) leaf and mesenchymal cell-on histological architecture of cardiac muscles. Stem cell therapy holds a great promise for the repair of injured tissues. Mesenchymal stem cells (MSCs) and M. oleifera leaf have the potential to present a new trend of treatment. This work targeted to ponder the impact of bone marrow-MSCs and aqueous extract of M.oleifera leaf on amiodarone-induced myocardial damage in albino rats by histological methods. Materials and methods Forty-two adult male albino rats were used. The bone marrow-MSCs source was seven rats. Thirty-five rats were divided into the following groups: negative control group included seven rats that received no treatment; vehicle control group included seven rats that received polysorbate 80; seven rats involved in group received amiodarone orally for every day for 5 weeks and also group treated with M. oleifera leaf extract included seven rats that received extract 2h before orally uptake amiodarone and stem cell-treated group included seven rats that received stem cells after amiodarone stoppage. Myocardium specimens were histologically examined. Results Amiodarone group showed disrupted myocardial architecture, lysis of myofibrils, and significant ultra-structural changes in myocardium in the form of disorganized myofibrils, swollen destructed mitochondria, SER dilatation, and cellular infiltration with mononuclear inflammatory cells. Minimal microscopic changes of the myocardium were obtained in Bone marrow-MSCs and natural extract of M. oleifera leaf treated groups with preservation of the normal structure of the cardiac myocytes. Conclusion Mesenchymal stem cells (MSCs) and M. oleifera leaf can improve the deleterious effects associated with amiodarone-induced myocardial damage. |
