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Prof. Ehab Mostafa El-Nahas Mohammed Nassar :: Publications: |
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| Title: | Isolation and Identification of Lumpy Skin Disease Virus from Naturally Infected Buffaloes at Kaluobia, Egypt |
| Authors: | El-Nahas, E.M.; El-Habbaa,A.S. ; El-bagoury, G.F. and Mervat E.I. Radwan |
| Year: | 2011 |
| Keywords: | Lumpy Skin Disease, Buffaloes, Isolation, Egypt |
| Journal: | Global Veterinaria |
| Volume: | 7 |
| Issue: | 3 |
| Pages: | 234-237 |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | Ehab Mostafa El-Nahas Mohammed Nassar _7 (1).PDF |
| Supplementary materials | Not Available |
| Abstract: |
Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected buffaloes at Kaluobia governorate. The virus was isolated on chorio-allantoic membrane (CAM) of embryonated chicken eggs (ECEs) and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against LSDV. Further investigations were carried out using electron microscopic (EM) examination and polymerase chain reaction (PCR) for attachment protein gene confirmed LSDV isolate with specific amplified product 192bp |
