Tl IE EGYPTIAN JOURNAL OF MEDICAL SCIENCES VOL. 26 (1) June 2005 ( Suppl. I) (ISSN: 1110-0540)
5. Egypt. J. Med. Sci. 26 (1) June 2005 (Suppl. 1): 67-77.
CORRELATION BETWEEN ANTIBODIES PATTERN AND ANTIGENEMIA IN FASCIOLIASIS
By
Aniany F. El-Fakahany, Hassan H. Hamadto, Ali. E. Ali,
Amina I. AbdcJ-Maboud, Azza S.El-Hamshary and Rabab F. Mohamed
Department of Parasitology, Faculty of Medicine, Banha University
ABSTRACT
This work was carried out on 30 Fasciola positive patients of both sexes, aged from 5-56 years old, 20 patients infected with other parasites than Fasciola and 20 healthy individu¬als (normal control). Detection of anti-Fasciola IgG, IgM, IgA and IgE anti¬bodies was done by indirect ELISA assay, Ami-rasclota IgG antibodies were detected in 93% of cases with sensitivity 93% and specificity 80%, IgM antibodies were detected in 83% of cases with sensitivity 83% and specificity 90%, IgE antibodies were detected in 33% of cases with sensitiv¬ity 33% and specificity 100% and IgA antibodies were detected in 70% of cases with sensitivity 70% and speci¬ficity 72.5%. By using competitive inhibition ELISA technique, Fasciola excretory / secretory (E/S) antigen was detected in 20% of cases with sensitiv¬ity 20% and specificity 92.5%. Both of anti-Fasciola IgG and IgM antibodies were detected in all Fasciola antigen positive cases. The highest specificity for Fasciola diagnosis assays was that of IgE, the lowest was that of IgA ELISA assay. It was concluded that the highest sensitivity for Fasciola di¬agnosis was that of anti-Fasciola IgG
ELISA and the lowest was that of competitive inhibition ELISA assay for detection of Fasciola antigen.
|
