Prof. Ayman Said Emam Desoky El-Habbaa :: Publications:

BVD virus is an important pathogen that infects cattle all over the world, causes important economic losses in cattle breeding. A preliminary trial for offering the effect of some plant extracts on cytopathogenicity and titer of BVD virus was carried out. BVD virus was titrated on MDBK cell culture 6.5 log10 TCID50/ml that was used for studying effect of curcumin and garlic extracts. Assay of toxicity of curcumin extract on MDBK cells showed that 103.25 mg/ml was lethal to MDBK cells as the lethal dose 50%. Screening of antiviral activity of Curcumin extract simultaneously added with the virus on MDBK cells showed that 25% of the cells developed CPE on 2nd day post inoculation (DPI), increased to affect 50% of the cells on 3rd DPI and 100% CPE was recorded on 4th DPI. Assay of toxicity of garlic extract on MDBK cells showed that 102.66 mg/ml was lethal to MDBK cells as the lethal dose 50%. Antiviral activity of garlic extract on MDBK cells simultaneously added with the virus on cells showed that 25% of the cells developed CPE on the 1st DPI, increased to affect 50% of the cells on 2nd DPI and 100% CPE was recorded on 3rd DPI for both cell culture treated with the extract and control untreated MDBK cells. It was concluded that curcumin extract reduced cytopathogenicity and titer of BVD virus affecting its replication