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Prof. Ayman Said Emam Desoky El-Habbaa :: Publications: |
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| Title: | Isolation And Molecular Identification Of Bovine Herpes Virus-1 Among Infected Buffaloes From Kalubia Governorate, Egypt |
| Authors: | AS El-Habbaa |
| Year: | 2012 |
| Keywords: | Bovine Herpes virus -1,Buffaloes, Kalubia, Egypt, |
| Journal: | Egyptian Journal Of Virology |
| Volume: | 9 |
| Issue: | 2 |
| Pages: | 465 – 476 |
| Publisher: | Egyptian Syndicate of Virology |
| Local/International: | Local |
| Paper Link: | Not Available |
| Full paper | Not Available |
| Supplementary materials | Not Available |
| Abstract: |
One hundred samples from clinically suspected buffaloes for Bovine Herpes virus -1 infection at Kalubia governorate were subjected for trials of virus isolation on MDBK cell line. Nine positive samples as follows 1 out of 25, 6 out of 25, 1 out of 25 and 1 out of 25 of ocular, nasal, vaginal swabs and tissue samples, respectively. Positive samples upon isolation were identified with indirect fluorescent antibody technique (IFAT) and revealed that 2, 1, and 1 were positive for nasal swabs, vaginal swabs and tissue samples respectively. Polymerase chain reaction (PCR) for glycoprotein B (gB) gene have also been used for molecular identification. |
