The objective of this study was to describe an alternative technique for opened castration in donkeys with minimal trauma and without any surgical incisions of the scrotum, through ligation of spermatic cord in situ. An experimental study was conducted on, fifteen mature donkeys (1-3 years old) at faculty of veterinary medicine at Moshtohour, Benha University. The animals were divided into three groups (5 donkeys for each). Percutaneous ligation of the spermatic cord using a sterile injectable needle 16 gauge in diameter and 6-10 cm in length was performed unilaterally in group 1 and bilaterally in group 2. The last group (3) was castrated by open traditional technique. Clinical observations were recorded. Scrotal and preputial dimensions were measured before and after ligation. The serum testosterone concentrations were evaluated pre and post-operation. The testes were collected from groups (1&2) 3 months post-ligation by opened castration for gross pathological and histopathological examination. Scrotal and preputial swellings were the only observed short-term complications. Results were revealed a significant reduction in the circumference and length of the scrotum and testicular atrophy post-ligation of spermatic cord. Significant reduction in basal serum testosterone levels 3 months after ligation than pre-ligation levels were observed. Hyalinization of the semineferous tubules and loss of testicular interstitial tissue were indicative for complete ischemic necrosis. It was concluded that, the technique was simple, quick and non-invasive performed without skin incision and with minimal complications and without need for post-operative care. |
