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Prof. Mahmoud El-Sayed Abed Abou-EL-Roos :: Publications: |
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| Title: | Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques |
| Authors: | AR Elkhawagah, V Longobardi, B Gasparrini, GA Sosa, G Bifulco, MEA Abouelroos, AE Abd el-ghafar, G Campanile |
| Year: | 2013 |
| Keywords: | Not Available |
| Journal: | Journal of Reproduction and Infertility |
| Volume: | 4 |
| Issue: | 3 |
| Pages: | 19-28 |
| Publisher: | IDOS Publication |
| Local/International: | International |
| Paper Link: | |
| Full paper | Not Available |
| Supplementary materials | Not Available |
| Abstract: |
Abstract: This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration |
