We performed fluorescent in situ hybridisation for loach Misgurnus anguillicaudatus bmi1, msi1
and sox2 genes, which are used as an indicator of the sites of multipotent stem cells. proliferating
cell nuclear antigen (PCNA), bromodeoxyuridine (BRDU) and KI67 markers were used as indicators of
proliferating cells and glial fibrillary acidic protein (GFAP) immunofluorescence was used for
detection of the glial property of cells, as well as, immunohistochemistry detected the role of
peroxisome proliferator-activated receptor (PPAR)α and γ in retinal neurogenesis. Our results
determined that the lens and the retina of loach M. anguillicaudatus contain proliferative and
pluripotent stem cells that have both glial and neuroepithelial properties, which add new cells
continuously throughout life even without injury-induced proliferation. The PPARα has an
essential function in providing energy supply for retinal neurogenesis more than PPARγ. |
