Background: Barrett's esophagus (BE) is an acquired
metaplastic lesion with unpredictable potential for esophageal
adenocarcinoma (EAC). Searching for immunohistochemical
markers for predicting progression in Barrett's esophagus is
of increasing interest.
Aim of Study: The aim of this study is to detect early
dysplastic changes in patients with BE for better management
of diagnosed cases.
Material and Methods: This retrospective study was
carried upon 28 endoscopic biopsies of BE; 6 cases of Barrett's
esophagus without dysplasia, 12 cases were Barrett's esophagus
with low grade dysplasia and 10 cases with high grade dysplasia. Cases were collected from archives of Pathology
Department and Early Cancer Detection Unit (ECDU), Faculty
of Medicine, Benha University during the years 2015-2020.
IMP3 and P53 immunohistochemichal staining were performed
and evaluated for each case.
Results: IMP3 was positive with high expression in 80%
of high grade dysplasia cases which was a statistically significant relation between IMP3 expression and grade of dysplasia.
P53 was a highly sensitive marker for early dysplastic changes,
reporting 100% sensitivity to low grade dysplasia. P53 was
also highly specific to high grade dysplasia (100%). IMP3
was found to be highly sensitive to high grade dysplastic
changes (90%).
Conclusion: Combination of both markers could be helpful
in detection of early dysplastic changes in Barrett's esophagus
patients who are at risk of malignancy to start a strict followup procedures. Both markers together could be useful in
detection ofhigh grade dysplasiaand rapid intervention to
prevent malignant transformation. |
