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Prof. Rajaa Mostafa El-Belashy :: Publications:

Title:
Exploiting biosynthetic gold nanoparticles for improving the aqueous solubility of metal-free phthalocyanine as biocompatible PDT agent
Authors: Shaimaa M.I. Alexeree a, Mahmoud A. Sliema, Ragaa M. EL-Balshy b, RehabM. Amina, M.A. Haritha,⁎ a National Institute of Laser Enhanced Science, Cairo University, Egypt b Department of Zoology, Faculty of Science, Benha University, Egypt
Year: 2017
Keywords: Biosynthesis Gold nanoparticles Metal-free phthalocyanine Pc-Au nanoconjugates
Journal: Materials Science and Engineering C 76 (2017) 727–734
Volume: 76
Issue: Not Available
Pages: 727-734
Publisher: M.A. Haritha,⁎
Local/International: International
Paper Link:
Full paper Rajaa Mostafa El-Belashy_Gold nanoparticles.pdf
Supplementary materials Not Available
Abstract:

Increasing the limit of dispersion of metal-free phthalocyanine (H2Pc) in an aqueous mediumusing biosynthetic gold nanoparticles for photodynamic therapy (PDT) is investigated. Gold nanoparticles (Au NPs) are biosynthesized in one step using Potatoes (Solanum tuberosum) extract and are characterized by UV/VIS spectrophotometry, Fourier transformer infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The metal-free phthalocyanine is conjugated to the surface of the gold nanoparticles in a side to side regime through the secondary amine groups of H2Pc. The clear violet solution of phthalocyanine-gold (Pc-Au) nanoconjugates is investigated by UV–VIS, FTIR and TEMtechniques. Disappearance of the absorption band of the secondary amine in the Pc-Au nanoconjugates compared to that of the parentH2Pc, and detection of the absorption band ofH2Pc in the aqueous mediumconfirmed the dispersion of H2Pc and consequently the loading of H2Pc on the surface of Au NPs. The cytotoxic effect of biosynthetic gold nanoparticles and Pc-Au nanoconjugates compared to chemically synthesized gold nanoparticles on buffalo epithelial cells has been studied in vitro. Interestingly, the results showed that the biosynthetic Au NPs as well as Pc-Au nanoconjugates have no effect on buffalo epithelial cells viability, which indicating their biocompatibility contrary to the chemically synthesized Au NPs. This work will open the door, for the first time, for using H2Pc suspended in water for PDT and other phototherapeutic applications.

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