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Dr. Noha Mohamed Mokhtar Ashry :: Publications:

Title:
Isolation and Characterization of Endophytic Bacteria Isolated from Legumes and Non-Legumes Plants in Egypt
Authors: Rashed A. Zaghloul, Hamed E. Abou-Aly, Taha A.Tewfike* and Noha M. Ashry
Year: 2016
Keywords: Root colonization, endophytic bacteria, celullase, pectinase, PGP Traits, IAA, Phosphate solubilizers.
Journal: JOURNAL OF PURE AND APPLIED MICROBIOLOGY
Volume: 10
Issue: 1
Pages: 277-290
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Noha Mohamed Mokhtar Ashry_phd.pdf
Supplementary materials Not Available
Abstract:

Endophytic bacterial isolates were isolated from roots, nodules, leaves and stems of faba bean ( Vicia faba ) , pea ( Pisum sativum ) , fenugreek ( Trigonella foenumgracum ) , lupine ( Lupinus spp.), common bean (Phaseolus vulgaris) and rice (Oryza sativa) at flowering stage. A total of 167 endophytic isolates were screened qualitative and quantitative for cellulase and pectinase activities. Result showed that more than 55 isolates out of 167 were able to produce cellulase and pectinase enzymes. Total of 55 isolates were screened as plant growth-promoters (PGP) traits and root colonization. The highest values of log CFU was 6.4x105 of isolate TN10 inside the roots of faba bean plant. Moreover, 12 endophytic isolates produced IAA more than 25μ/ml in the presence of the precursor tryptophan. Also TN12, HN32 and RN62 isolates recorded the highest values of siderophores production. About 82% of the isolates showed positive results of HCN, 44 isolates were able to produce ammonia. The phosphate solubilization efficiency percentage were detected for endophytic isolates, 19 isolates showed the maximum range of phosphate solubilization efficiency (SE).

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