Dr. Abdelmotaleb Ahmed Elokil :: Publications:

Molecular analysis is an easier means to identify and isolate a specific gene which has imperative function for growth, body composition, fat deposition, metabolic and skeletal traits as well as the molecular genetics selection on individual genes is a very efficient method to genetically improve economically important traits in chickens. Insulin-like growth factor 1 (IGF- 1) is a member of a heterogeneous group of peptides with important growth-promoting effects in vitro as well as in vivo. It plays a fundamental role in postnatal chicken growth as a major mediator through which growth hormone exerts its biological effects. In this study, total RNA was isolated from (Cobb 500 breed) liver samples at 50 weeks age from national poultry company in Egypt. cDNA was produced by reverse transcriptase-polymerase chain reaction (RT-PCR). Standard PCR was carried on using two flanking primers containing HindIII and EcoRI restriction enzymes to generate the whole length of IGF-1 gene. A PCR product of 490bp, including the open reading frame of IGF-1 gene corresponding to the expected theoretical product size, was successfully amplified and double digested with HindIII and EcoR1 and cloned into same Restriction enzymes digested PCR3.1 mammalian expression vector. Interestingly, the cloned sequence was confirmed by DNA sequencing which reveals that, it is cloned in frame position and 99% matching except one nucleotide due to the heterozygous of isolated IGF-1 cDNA. In the present study we report the isolation, molecular cloning and sequencing of full-length IGF-1 cDNA from chicken liver, encoding the IGF-1 protein, can be used as a candidate gene in transgenic Egyptian native breed production based on the IGF-1 protein of exotic high producing chicken population and may give a tool in continuous improvements of the chicken industry concerning a native breed in Egypt.